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Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes

We have used proteinase K as a probe to detect cytoplasmically and luminally exposed segments of nascent polypeptides undergoing transport across mammalian microsomal membranes. A series of translocation intermediates consisting of discrete-sized nascent chains was prepared by including microsomal m...

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Bibliografiske detaljer
Format: Artigo
Sprog:Inglês
Udgivet: The Rockefeller University Press 1989
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2115410/
https://ncbi.nlm.nih.gov/pubmed/2537313
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