Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli.

A 13.6-kilobase (kb) Sau3AI restriction endonuclease fragment of Clostridium acetobutylicum DNA cloned into pBR322 enabled Escherichia coli ato mutants to grow on butyrate as a sole carbon source (But+). Complementation of the ato defect by the recombinant plasmid pJC6 was due to expression of the g...

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Publié dans:	J Bacteriol
Auteurs principaux:	Cary, J W, Petersen, D J, Papoutsakis, E T, Bennett, G N
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1988
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211500/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2844725/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.10.4613-4618.1988
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