Cloning and expression of Clostridium acetobutylicum phosphotransbutyrylase and butyrate kinase genes in Escherichia coli.

A 13.6-kilobase (kb) Sau3AI restriction endonuclease fragment of Clostridium acetobutylicum DNA cloned into pBR322 enabled Escherichia coli ato mutants to grow on butyrate as a sole carbon source (But+). Complementation of the ato defect by the recombinant plasmid pJC6 was due to expression of the g...

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Hlavní autoři: Cary, J W, Petersen, D J, Papoutsakis, E T, Bennett, G N
Médium: Artigo
Jazyk:Inglês
Vydáno: 1988
Témata:
Research Article
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC211500/
https://ncbi.nlm.nih.gov/pubmed/2844725
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