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Secretory protein translocation in a yeast cell-free system can occur posttranslationally and requires ATP hydrolysis

We describe an in vitro system with all components derived from the yeast Saccharomyces cerevisiae that can translocate a yeast secretory protein across microsomal membranes. In vitro transcribed prepro-alpha- factor mRNA served to program a membrane-depleted yeast translation system. Translocation...

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Bibliografski detalji
Format: Artigo
Jezik:Inglês
Izdano: The Rockefeller University Press 1986
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2114218/
https://ncbi.nlm.nih.gov/pubmed/3517001
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