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Tubulin hooks as probes for microtubule polarity: an analysis of the method and an evaluation of data on microtubule polarity in the mitotic spindle
The structural polarity of cellular microtubules can be visualized in situ by lysing cells in special buffers containing tubulin. Under these conditions, the tubulin polymerizes to form curved sheets which attach to the walls of the endogenous microtubules. When such decorated microtubules are cut i...
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| Natura: | Artigo |
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| Lingua: | Inglês |
| Pubblicazione: |
The Rockefeller University Press
1984
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2113111/ https://ncbi.nlm.nih.gov/pubmed/6693493 |
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