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Detection of actin assembly by fluorescence energy transfer

Fluorescence energy transfer was used to measure the assembly and disassembly of actin filaments. Actin was labeled at cysteine 373 with an energy donor (5-iodoacetamidofluorescein) or an energy acceptor (tetramethylrhodamine iodoacetamide or eosin iodoacetamide). Donor- labeled actin and acceptor-l...

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Format: Artigo
Sprache:Inglês
Veröffentlicht: The Rockefeller University Press 1981
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2111691/
https://ncbi.nlm.nih.gov/pubmed/6894758
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