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Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114.

The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Kalmokoff, M L, Jarrell, K F, Koval, S F
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211027/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3127380/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.4.1752-1758.1988
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