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Isolation of flagella from the archaebacterium Methanococcus voltae by phase separation with Triton X-114.

The flagella of Methanococcus voltae were isolated by using three procedures. Initially, cells were sheared to release the filaments, which were purified by differential centrifugation and banding in KBr gradients. Flagella were also prepared by solubilization of cells with 1% (vol/vol) Triton X-100...

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Detalles Bibliográficos
Publicado en:J Bacteriol
Principais autores: Kalmokoff, M L, Jarrell, K F, Koval, S F
Formato: Artigo
Idioma:Inglês
Publicado: American Society for Microbiology (ASM) 1988
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Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC211027/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3127380/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.4.1752-1758.1988
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