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Individual microtubules viewed by immunofluorescence and electron microscopy in the same PtK2 cell

PtK2 cells were grown on gold grids and treated with Triton X-100 in a microtubule stabilizing buffer. The resulting cytoskeletons were fixed with glutaraldehyde and subjected to the indirect immunofluorescence procedure using monospecific tubulin antibodies. Grids were examined first by fluorescenc...

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書目詳細資料
Main Authors: Osborn, M., Webster, R. E., Weber, K.
格式: Artigo
語言:Inglês
出版: The Rockefeller University Press 1978
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2110159/
https://ncbi.nlm.nih.gov/pubmed/567223
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