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ISOLATION OF CYTOPLASMIC AND CHLOROPLAST RIBOSOMES AND THEIR DISSOCIATION INTO ACTIVE SUBUNITS FROM CHLAMYDOMONAS REINHARDTII

A mixture of cytoplasmic (80S) and chloroplast (70S) ribosomes from Chlamydomonas reinhardtii was freed of contaminating membranes by sedimentation of the postmitochondrial supernatant through a layer of 1.87 M sucrose. The purified ribosomes were separated into 80S and 70S fractions by centrifugati...

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Detalhes bibliográficos
Main Authors: Chua, Nam-Hai, Blobel, G., Siekevitz, P.
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1973
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2109006/
https://ncbi.nlm.nih.gov/pubmed/4698907
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