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Simplified Procedure for the Isolation of Intact Chloroplasts from Chlamydomonas reinhardtii

A simple procedure that yields highly purified intact chloroplasts from Chlamydomonas reinhardtii is described. This procedure involves breakage of cell wall-deficient cells by passing them through a narrow bore syringe needle. The intact chloroplasts are then purified from the crude homogenate by d...

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Main Authors: Mason, Catherine B., Matthews, Sharon, Bricker, Terry M., Moroney, James V.
Formato: Artigo
Idioma:Inglês
Publicado: 1991
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC1081203/
https://ncbi.nlm.nih.gov/pubmed/16668587
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