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RIBOSOME-MEMBRANE INTERACTION : Nondestructive Disassembly of Rat Liver Rough Microsomes into Ribosomal and Membranous Components

In a medium of high ionic strength, rat liver rough microsomes can be nondestructively disassembled into ribosomes and stripped membranes if nascent polypeptides are discharged from the bound ribosomes by reaction with puromycin. At 750 mM KCl, 5 mM MgCl(2), 50 mM Tris·HCl, pH 7 5, up to 85% of all...

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Autors principals: Adelman, M. R., Sabatini, David D., Blobel, Günter
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1973
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108843/
https://ncbi.nlm.nih.gov/pubmed/4682341
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