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RIBOSOME-MEMBRANE INTERACTION : Nondestructive Disassembly of Rat Liver Rough Microsomes into Ribosomal and Membranous Components

In a medium of high ionic strength, rat liver rough microsomes can be nondestructively disassembled into ribosomes and stripped membranes if nascent polypeptides are discharged from the bound ribosomes by reaction with puromycin. At 750 mM KCl, 5 mM MgCl(2), 50 mM Tris·HCl, pH 7 5, up to 85% of all...

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書誌詳細
主要な著者: Adelman, M. R., Sabatini, David D., Blobel, Günter
フォーマット: Artigo
言語:Inglês
出版事項: The Rockefeller University Press 1973
主題:
オンライン・アクセス:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108843/
https://ncbi.nlm.nih.gov/pubmed/4682341
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