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FRACTIONATION OF THE NUCLEUS BY DIVALENT CATIONS : Isolation of Nuclear Membranes
0.3–0.5 M MgCl(2) was used to disassemble nuclei and to isolate by a single centrifugation in less than 3 hr a nuclear envelope fraction in 55–60% yield as assessed by phospholipid recovery. Its gross chemical composition was determined and its morphology was studied electron microscopically by sect...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The Rockefeller University Press
1972
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2108744/ https://ncbi.nlm.nih.gov/pubmed/4120072 |
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