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FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed...

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Detalhes bibliográficos
Main Authors: Plattner, Helmut, Fischer, Walter M., Schmitt, Werner W., Bachmann, Luis
Formato: Artigo
Idioma:Inglês
Publicado em: The Rockefeller University Press 1972
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://ncbi.nlm.nih.gov/pubmed/4622424
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