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FREEZE ETCHING OF CELLS WITHOUT CRYOPROTECTANTS

The technique of spray-freeze etching was applied to unicellular organisms. The superior freezing rates obtainable with this method gave excellent cryofixation on Chlorella, Euglena, and spermatozoa without the use of antifreeze agents, and cell damage due to ice crystal formation was never observed...

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Bibliografski detalji
Glavni autori: Plattner, Helmut, Fischer, Walter M., Schmitt, Werner W., Bachmann, Luis
Format: Artigo
Jezik:Inglês
Izdano: The Rockefeller University Press 1972
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2108701/
https://ncbi.nlm.nih.gov/pubmed/4622424
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