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CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS : I. Location of the Polypeptides within Ribosomes

Free ribosomes containing nascent polypeptide chains labeled in vitro were submitted to proteolysis at 0° by a mixture of trypsin and chymotrypsin. Sucrose gradient analysis showed that polysome patterns are retained even after 24 hr of proteolysis in the cold, while messenger RNA-free ribosomes (ge...

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Détails bibliographiques
Auteurs principaux: Blobel, G., Sabatini, D. D.
Format: Artigo
Langue:Inglês
Publié: The Rockefeller University Press 1970
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107986/
https://ncbi.nlm.nih.gov/pubmed/5458992
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