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CONTROLLED PROTEOLYSIS OF NASCENT POLYPEPTIDES IN RAT LIVER CELL FRACTIONS : II. Location of the Polypeptides in Rough Microsomes

Rough microsomes were incubated in an in vitro amino acid-incorporating system for labeling the nascent polypeptide chains on the membrane-bound ribosomes. Sucrose density gradient analysis showed that ribosomes did not detach from the membranes during incorporation in vitro. Trypsin and chymotrypsi...

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Detalles Bibliográficos
Main Authors: Sabatini, D. D., Blobel, G.
Formato: Artigo
Idioma:Inglês
Publicado: The Rockefeller University Press 1970
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Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107985/
https://ncbi.nlm.nih.gov/pubmed/5458993
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