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THE ISOLATION OF A MAJOR STRUCTURAL ELEMENT OF THE SEA URCHIN FERTILIZATION MEMBRANE

Procedures for isolating the contents of the cortical granules from the ova of the sea urchin, Strongylocentrotus purpuratus, are reported. Dithiothreitol is used to remove the vitelline coat; the "demembranated" eggs are then subsequently activated with butyric acid. By means of these pro...

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Bibliografiske detaljer
Hovedforfatter: Bryan, Joseph
Format: Artigo
Sprog:Inglês
Udgivet: The Rockefeller University Press 1970
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC2107967/
https://ncbi.nlm.nih.gov/pubmed/4190068
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