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THE ISOLATION OF A MAJOR STRUCTURAL ELEMENT OF THE SEA URCHIN FERTILIZATION MEMBRANE
Procedures for isolating the contents of the cortical granules from the ova of the sea urchin, Strongylocentrotus purpuratus, are reported. Dithiothreitol is used to remove the vitelline coat; the "demembranated" eggs are then subsequently activated with butyric acid. By means of these pro...
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| Main Author: | |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
The Rockefeller University Press
1970
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2107967/ https://ncbi.nlm.nih.gov/pubmed/4190068 |
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