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ISOLATION OF SKELETAL MUSCLE NUCLEI

A method employing aqueous media for isolation of nuclei from rat skeletal muscle is described. The technique involves (a) mincing and then homogenizing in a 0.32 M sucrose-salt solution with a Potter-Elvehjem type homogenizer using a Delrin (an acetal resin) pestle and a carefully controlled, relat...

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Autors principals: Edelman, Jean C., Edelman, P. Michael, Knigge, Karl M., Schwartz, Irving L.
Format: Artigo
Idioma:Inglês
Publicat: The Rockefeller University Press 1965
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2106724/
https://ncbi.nlm.nih.gov/pubmed/4287141
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