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Use of lac fusions to measure in vivo regulation of expression of Escherichia coli proton-translocating ATPase (unc) genes.

In-frame fusions to lacZ were constructed in two adjacent genes of the unc operon of Escherichia coli, uncA and uncG, which code for the alpha and gamma subunits of the proton-translocating ATPase. After each fusion was moved into the E. coli chromosome, measurement of beta-galactosidase activities...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Angov, E, Brusilow, W S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1988
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210668/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2891682/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.1.459-462.1988
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