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Use of lac fusions to measure in vivo regulation of expression of Escherichia coli proton-translocating ATPase (unc) genes.

In-frame fusions to lacZ were constructed in two adjacent genes of the unc operon of Escherichia coli, uncA and uncG, which code for the alpha and gamma subunits of the proton-translocating ATPase. After each fusion was moved into the E. coli chromosome, measurement of beta-galactosidase activities...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Angov, E, Brusilow, W S
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1988
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210668/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2891682/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.1.459-462.1988
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