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Isolation of Salmonella typhimurium cys genes by transduction with a library of recombinant plasmids packaged in bacteriophage P22HT capsids.

We have prepared a library of Salmonella typhimurium genomic fragments cloned in pBR322 and packaged in P22HT capsids. Plasmids carrying 24 of 26 specific genes searched for were isolated by transduction at frequencies of 1 to 344 per 10(6) plasmid transductants. All 11 known genes of the cysteine r...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Monroe, R S, Kredich, N M
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1988
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210603/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/3275623/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.170.1.42-47.1988
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