Transformation of the archaebacterium Halobacterium volcanii with genomic DNA.

We describe optimization of a transformation system for the halophilic archaebacterium Halobacterium volcanii. Transformation of spheroplasts in the presence of polyethylene glycol permits the uptake and expression of high-molecular-weight linear fragments of genomic DNA as well as plasmid or bacter...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Cline, S W, Schalkwyk, L C, Doolittle, W F
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210307/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2768194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4987-4991.1989
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