Transformation of the archaebacterium Halobacterium volcanii with genomic DNA.
We describe optimization of a transformation system for the halophilic archaebacterium Halobacterium volcanii. Transformation of spheroplasts in the presence of polyethylene glycol permits the uptake and expression of high-molecular-weight linear fragments of genomic DNA as well as plasmid or bacter...
Uloženo v:
| Vydáno v: | J Bacteriol |
|---|---|
| Hlavní autoři: | , , |
| Médium: | Artigo |
| Jazyk: | Inglês |
| Vydáno: |
American Society for Microbiology (ASM)
1989
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| Témata: | |
| On-line přístup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210307/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2768194/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4987-4991.1989 |
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