Transformation of a conditionally peptidoglycan-deficient mutant of Staphylococcus aureus with plasmid DNA.
A simple and reliable method for polyethylene glycol-induced plasmid transformation of a temperature-sensitive peptidoglycan-deficient mutant of Staphylococcus aureus is described. The procedure uses strains carrying the tofA372 mutation grown under conditions that yield osmotically fragile cells ca...
Αποθηκεύτηκε σε:
| Εκδόθηκε σε: | J Bacteriol |
|---|---|
| Κύριοι συγγραφείς: | , |
| Μορφή: | Artigo |
| Γλώσσα: | Inglês |
| Έκδοση: |
American Society for Microbiology (ASM)
1989
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| Θέματα: | |
| Διαθέσιμο Online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210296/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2549004/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4906-4913.1989 |
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