Transformation of a conditionally peptidoglycan-deficient mutant of Staphylococcus aureus with plasmid DNA.

A simple and reliable method for polyethylene glycol-induced plasmid transformation of a temperature-sensitive peptidoglycan-deficient mutant of Staphylococcus aureus is described. The procedure uses strains carrying the tofA372 mutation grown under conditions that yield osmotically fragile cells ca...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Nieuwlandt, D T, Pattee, P A
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1989
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210296/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2549004/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4906-4913.1989
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