Llwytho...

New method for generating deletions and gene replacements in Escherichia coli.

We describe a method for generating gene replacements and deletions in Escherichia coli. The technique is simple and rapid and can be applied to most genes, even those that are essential. What makes this method unique and particularly effective is the use of a temperature-sensitive pSC101 replicon t...

Disgrifiad llawn

Wedi'i Gadw mewn:
Manylion Llyfryddiaeth
Prif Awduron: Hamilton, C M, Aldea, M, Washburn, B K, Babitzke, P, Kushner, S R
Fformat: Artigo
Iaith:Inglês
Cyhoeddwyd: 1989
Pynciau:
Mynediad Ar-lein:https://ncbi.nlm.nih.gov/pmc/articles/PMC210259/
https://ncbi.nlm.nih.gov/pubmed/2548993
Tagiau: Ychwanegu Tag
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