Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane.
When a signal sequence is attached to beta-galactosidase, the normally cytoplasmic protein is unable to fully traverse the cytoplasmic membrane. We used a genetic approach to study those features of beta-galactosidase responsible for the block in translocation. By using both in vivo and in vitro tec...
Guardado en:
| Publicado en: | J Bacteriol |
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| Autores principales: | , , , , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Microbiology (ASM)
1989
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| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210258/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2527843/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4609-4616.1989 |
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