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Genetic studies on the inability of beta-galactosidase to be translocated across the Escherichia coli cytoplasmic membrane.

When a signal sequence is attached to beta-galactosidase, the normally cytoplasmic protein is unable to fully traverse the cytoplasmic membrane. We used a genetic approach to study those features of beta-galactosidase responsible for the block in translocation. By using both in vivo and in vitro tec...

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Dades bibliogràfiques
Publicat a:J Bacteriol
Autors principals: Lee, C, Li, P, Inouye, H, Brickman, E R, Beckwith, J
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology (ASM) 1989
Matèries:
Accés en línia:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210258/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2527843/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.9.4609-4616.1989
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