Cloning and sequencing of the gene encoding a 125-kilodalton surface-layer protein from Bacillus sphaericus 2362 and of a related cryptic gene.

Using the vector pGEM-4-blue, a 4,251-base-pair DNA fragment containing the gene for the surface (S)-layer protein of Bacillus sphaericus 2362 was cloned into Escherichia coli. Determination of the nucleotide sequence indicated an open reading frame (ORF) coding for a protein of 1,176 amino acids wi...

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Détails bibliographiques

Publié dans:	J Bacteriol
Auteurs principaux:	Bowditch, R D, Baumann, P, Yousten, A A
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 1989
Sujets:	Research Article
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210188/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2666389/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.8.4178-4188.1989
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