Cloning and sequencing of the gene encoding a 125-kilodalton surface-layer protein from Bacillus sphaericus 2362 and of a related cryptic gene.

Using the vector pGEM-4-blue, a 4,251-base-pair DNA fragment containing the gene for the surface (S)-layer protein of Bacillus sphaericus 2362 was cloned into Escherichia coli. Determination of the nucleotide sequence indicated an open reading frame (ORF) coding for a protein of 1,176 amino acids wi...

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Bibliographic Details
Main Authors: Bowditch, R D, Baumann, P, Yousten, A A
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC210188/
https://ncbi.nlm.nih.gov/pubmed/2666389
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