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Two Gears of Pumping by the Sodium Pump

The kinetics of the phosphorylation and subsequent conformational change of Na(+),K(+)-ATPase was investigated via the stopped-flow technique using the fluorescent label RH421 (pH 7.4, 24°C). The enzyme was preequilibrated in buffer containing 130 mM NaCl to stabilize the E1(Na(+))(3) state. On mixi...

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Bibliografske podrobnosti
Main Authors: Clarke, Ronald J., Kane, David J.
Format: Artigo
Jezik:Inglês
Izdano: The Biophysical Society 2007
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2098724/
https://ncbi.nlm.nih.gov/pubmed/17766357
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.107.111591
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