Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system.

The transposons Tn916 and Tn4001 and a series of integrating plasmids derived from their antibiotic resistance genes were used to examine polyethylene glycol-mediated transformation of Mycoplasma pulmonis. Under optimal conditions, Tn916 and Tn4001 could be introduced into M. pulmonis at frequencies...

Täydet tiedot

Bibliografiset tiedot

Julkaisussa:	J Bacteriol
Päätekijät:	Mahairas, G G, Minion, F C
Aineistotyyppi:	Artigo
Kieli:	Inglês
Julkaistu:	American Society for Microbiology (ASM) 1989
Aiheet:	Research Article
Linkit:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209821/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2539351/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.4.1775-1780.1989
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