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Transformation of Mycoplasma pulmonis: demonstration of homologous recombination, introduction of cloned genes, and preliminary description of an integrating shuttle system.

The transposons Tn916 and Tn4001 and a series of integrating plasmids derived from their antibiotic resistance genes were used to examine polyethylene glycol-mediated transformation of Mycoplasma pulmonis. Under optimal conditions, Tn916 and Tn4001 could be introduced into M. pulmonis at frequencies...

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Bibliografiske detaljer
Udgivet i:J Bacteriol
Principais autores: Mahairas, G G, Minion, F C
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology (ASM) 1989
Fag:
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209821/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2539351/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.4.1775-1780.1989
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