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Cyclic-AMP-dependent switch in initiation of transcription from the two promoters of the Escherichia coli gal operon: identification and assay of 5'-triphosphate ends of mRNA by GTP:RNA guanyltransferase.

We have studied the initiation of transcription of the gal operon in Escherichia coli (i) by analyzing the 5'-triphosphate ends and (ii) by measuring the level of promoter-proximal gal mRNA made in vivo. The 5' termini were identified and quantified by capping with GTP:mRNA guanyltransferase, and th...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Irani, M, Musso, R, Adhya, S
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209790/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2537823/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.3.1623-1630.1989
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