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Construction and shuttling of novel bifunctional vectors for Streptomyces spp. and Escherichia coli.

Shuttle vectors for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of an artificial multicopy E. coli replicon and DNA fragments of pIJ702. Stable transfer to Streptomyces lividans was obtained. Marked differences in transformation efficiency were observ...

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Bibliographic Details
Published in:J Bacteriol
Main Authors: Neesen, K, Volckaert, G
Format: Artigo
Language:Inglês
Published: American Society for Microbiology (ASM) 1989
Subjects:
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209782/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2646288/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.3.1569-1573.1989
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