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Construction and shuttling of novel bifunctional vectors for Streptomyces spp. and Escherichia coli.

Shuttle vectors for gene transfer between Streptomyces spp. and Escherichia coli have been constructed by fusion of an artificial multicopy E. coli replicon and DNA fragments of pIJ702. Stable transfer to Streptomyces lividans was obtained. Marked differences in transformation efficiency were observ...

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Detalhes bibliográficos
Publicado no:J Bacteriol
Principais autores: Neesen, K, Volckaert, G
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 1989
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209782/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2646288/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.3.1569-1573.1989
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