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Site-directed mutation of the Escherichia coli ada gene: effects of substitution of methyl acceptor cysteine-321 by histidine in Ada protein.

Oligodeoxynucleotide-mediated mutagenesis of the ada gene of Escherichia coli was used to produce two mutant Ada proteins. In mutant I the methyl acceptor Cys-321 for O6-methylguanine was replaced by histidine; and in mutant II the positions of Cys-321 and His-322 of the wild-type protein were inver...

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Detalhes bibliográficos
Main Authors: Tano, K, Bhattacharyya, D, Foote, R S, Mural, R J, Mitra, S
Formato: Artigo
Idioma:Inglês
Publicado em: 1989
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC209778/
https://ncbi.nlm.nih.gov/pubmed/2493448
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