Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.

The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the...

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Pubblicato in:	J Bacteriol
Autori principali:	Saito, T, Suzuki, K, Yamamoto, J, Fukui, T, Miwa, K, Tomita, K, Nakanishi, S, Odani, S, Suzuki, J, Ishikawa, K
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1989
Soggetti:	Research Article
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209571/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2644188/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.1.184-189.1989
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