Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.

The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the...

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Autores principales: Saito, T, Suzuki, K, Yamamoto, J, Fukui, T, Miwa, K, Tomita, K, Nakanishi, S, Odani, S, Suzuki, J, Ishikawa, K
Formato: Artigo
Lenguaje:Inglês
Publicado: 1989
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Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC209571/
https://ncbi.nlm.nih.gov/pubmed/2644188
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