Cloning, nucleotide sequence, and expression in Escherichia coli of the gene for poly(3-hydroxybutyrate) depolymerase from Alcaligenes faecalis.

The extracellular poly(3-hydroxybutyrate) depolymerase gene from Alcaligenes faecalis T1 was cloned into Escherichia coli DH1 by using the plasmid pUC8. An A. faecalis T1 genomic library was prepared in E. coli from a partial Sau3AI digest and screened with antibody against the depolymerase. Of the...

Full description

Saved in:
Bibliographic Details
Main Authors: Saito, T, Suzuki, K, Yamamoto, J, Fukui, T, Miwa, K, Tomita, K, Nakanishi, S, Odani, S, Suzuki, J, Ishikawa, K
Format: Artigo
Language:Inglês
Published: 1989
Subjects:
Research Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC209571/
https://ncbi.nlm.nih.gov/pubmed/2644188
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items