Overproduction, purification, and characterization of Bacillus subtilis RNA polymerase sigma A factor.
By use of a T7 expression system, large amounts of active Bacillus subtilis RNA polymerase sigma A factor were produced in Escherichia coli cells. This overproduced protein was found in the form of inclusion bodies and constituted 40% of the total cellular protein. Because of the ease of isolation o...
Enregistré dans:
| Publié dans: | J Bacteriol |
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| Auteurs principaux: | , |
| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
American Society for Microbiology (ASM)
1990
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209133/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2111806/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.6.3257-3263.1990 |
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