Overproduction, purification, and characterization of Bacillus subtilis RNA polymerase sigma A factor.

By use of a T7 expression system, large amounts of active Bacillus subtilis RNA polymerase sigma A factor were produced in Escherichia coli cells. This overproduced protein was found in the form of inclusion bodies and constituted 40% of the total cellular protein. Because of the ease of isolation o...

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Detalhes bibliográficos

Publicado no:	J Bacteriol
Principais autores:	Chang, B Y, Doi, R H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1990
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209133/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2111806/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.6.3257-3263.1990
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