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Overproduction, purification, and characterization of Bacillus subtilis RNA polymerase sigma A factor.

By use of a T7 expression system, large amounts of active Bacillus subtilis RNA polymerase sigma A factor were produced in Escherichia coli cells. This overproduced protein was found in the form of inclusion bodies and constituted 40% of the total cellular protein. Because of the ease of isolation o...

Täydet tiedot

Tallennettuna:
Bibliografiset tiedot
Päätekijät: Chang, B Y, Doi, R H
Aineistotyyppi: Artigo
Kieli:Inglês
Julkaistu: 1990
Aiheet:
Linkit:https://ncbi.nlm.nih.gov/pmc/articles/PMC209133/
https://ncbi.nlm.nih.gov/pubmed/2111806
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