Insertional mutagenesis of a plasmid-borne Escherichia coli rpoB gene reveals alterations that inhibit beta-subunit assembly into RNA polymerase.

A plasmid was constructed that overproduces the Escherichia coli RNA polymerase beta subunit from a lac promoter-rpoB fusion. The overproduced, plasmid-encoded beta subunit assembled into functional RNA polymerase that supplied greater than 90% of the transcriptional capacity of the cells. Excess be...

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Veröffentlicht in:	J Bacteriol
Hauptverfasser:	Landick, R, Colwell, A, Stewart, J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	American Society for Microbiology (ASM) 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC209080/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2160927/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.6.2844-2854.1990
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