Expression of cloned rpoB gene of Escherichia coli: a genetic system for the isolation of dominant negative mutations and overproduction of defective beta subunit of RNA polymerase.

The rifampin resistance rifD18 allele of rpoB, carried on the expression plasmid pXT7 beta, is controlled by a strong bacteriophage T7 late promoter and two weak Escherichia coli promoters. Depending on the host strain, pXT7 beta specifies different levels of Rifr beta subunit, providing a system fo...

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Detalles Bibliográficos

Publicado en:	J Bacteriol
Principais autores:	Lee, J Y, Zalenskaya, K, Shin, Y K, McKinney, J D, Park, J H, Goldfarb, A
Formato:	Artigo
Idioma:	Inglês
Publicado:	American Society for Microbiology (ASM) 1989
Assuntos:	Research Article
Acceso en liña:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC210007/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2656636/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.171.6.3002-3007.1989
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