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A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions
Prions are usually quantified by bioassays based on intracerebral inoculation of mice that are slow, imprecise, and costly. We have isolated neuroblastoma N2a sublines highly susceptible to mouse prions, as evidenced by accumulation of infectivity and the scrapie form of prion protein (PrP(Sc)), and...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
National Academy of Sciences
2003
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC208815/ https://ncbi.nlm.nih.gov/pubmed/14504404 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1834432100 |
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