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A quantitative, highly sensitive cell-based infectivity assay for mouse scrapie prions

Prions are usually quantified by bioassays based on intracerebral inoculation of mice that are slow, imprecise, and costly. We have isolated neuroblastoma N2a sublines highly susceptible to mouse prions, as evidenced by accumulation of infectivity and the scrapie form of prion protein (PrP(Sc)), and...

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Bibliografiska uppgifter
Huvudupphovsmän: Klöhn, P.-C., Stoltze, L., Flechsig, E., Enari, M., Weissmann, C.
Materialtyp: Artigo
Språk:Inglês
Publicerad: National Academy of Sciences 2003
Ämnen:
Länkar:https://ncbi.nlm.nih.gov/pmc/articles/PMC208815/
https://ncbi.nlm.nih.gov/pubmed/14504404
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1834432100
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