Mutagenesis of Bordetella pertussis with transposon Tn5tac1: conditional expression of virulence-associated genes.
The Tn5tac1 transposon contains a strong outward-facing promoter, Ptac, a lacI repressor gene, and a selectable Kanr gene. Transcription from Ptac is repressed by the lacI protein unless an inducer (isopropyl-beta-D-thiogalactopyranoside [IPTG]) is present. Thus, Tn5tac1 generates insertion mutation...
Zapisane w:
| Wydane w: | J Bacteriol |
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| Główni autorzy: | , , |
| Format: | Artigo |
| Język: | Inglês |
| Wydane: |
American Society for Microbiology (ASM)
1990
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| Hasła przedmiotowe: | |
| Dostęp online: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208656/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2156797/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.4.1681-1687.1990 |
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