Nalaganje...
Mutagenesis of Bordetella pertussis with transposon Tn5tac1: conditional expression of virulence-associated genes.
The Tn5tac1 transposon contains a strong outward-facing promoter, Ptac, a lacI repressor gene, and a selectable Kanr gene. Transcription from Ptac is repressed by the lacI protein unless an inducer (isopropyl-beta-D-thiogalactopyranoside [IPTG]) is present. Thus, Tn5tac1 generates insertion mutation...
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| Main Authors: | , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1990
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC208656/ https://ncbi.nlm.nih.gov/pubmed/2156797 |
| Oznake: |
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