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Improved vector system for constructing transcriptional fusions that ensures independent translation of lacZ.

An improved vector system has been developed for the in vitro construction of transcriptional fusions to lacZ. The principal feature is an RNaseIII cleavage site inserted between the polylinker cloning site and the promoterless lacZ gene. When these vectors are used to construct transcriptional fusi...

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Autors principals: Linn, T, St Pierre, R
Format: Artigo
Idioma:Inglês
Publicat: 1990
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC208539/
https://ncbi.nlm.nih.gov/pubmed/2137119
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