A simple and sensitive in vivo luciferase assay for tRNA-mediated nonsense suppression.
We present a rapid assay for tRNA suppression in living Escherichia coli. An amber, ochre, or opal nonsense mutation in a cloned luxB gene from the bacterium Vibrio harveyi was suppressed. Because luciferase (Lux) activity depends completely on the appearance of the full-length luxB gene product, th...
Guardat en:
| Publicat a: | J Bacteriol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
American Society for Microbiology (ASM)
1990
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC208482/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2105299/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.172.2.595-602.1990 |
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